Cot-based cloning and sequencing of the short arm of wheat chromosome 1B

نویسندگان

  • H. Šimková
  • J. Janda
  • E. Hřibová
  • J. Šafář
  • J. Doležel
چکیده

Sequencing of cereal genomes is not a feasible task due to their large size and high content of repetitive DNA sequences. There are two basic approaches to simplify analysis of such genomes: reduced representation approaches, such as EST sequencing, methyl filtration and Cot-based cloning and sequencing; on the other side there is analysis of genomes in a step-wise manner, e.g. through creation of chromosome-specific genomic resources. Combination of both approaches – i.e. Cot-based cloning and sequencing of DNA obtained from a chromosome-arm-specific BAC library – was tested in this work.

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تاریخ انتشار 2005